DM50014 | Alkaline Peptone Water

CAS Number

Formula

Weight

Usages:
For selective cultivation of bacteria especially Vibrio .
 
Principle:
Peptone provide carbon and nitrogen sources; sodium chloride to maintain osmotic equilibrium; higher pH can suppress the growth of coliform and other bacteria, it is conducive to the growth of Vibrio cholerae.
 
Formulation(per liter):
Peptone 10g
Sodium chloride 10g
Final pH 8.5 ± 0.2
 
How to use:
1.Suspend 18g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
 
Quality control:
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Item The name and number of strain Growth Colony Color
1 Vibrio cholerae VbO Good Turbid broth
2 Escherichia coli ATCC25922 Poor --
 

 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

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