Products

Principle

The media is composed of peptone, tryptone, yeast extract, meat extract, dextrose and agar. Peptone, tryptone, yeast extract and meat extract provide nitrogen, vitamins essential nutrients. Dextrose serves as energy source. Agar is solidifying agent. This medium is used as seed agar, maintenance agar for the different variety of test microbes. Antibiotic assay medium A is excellent medium for antibiotic diffusion and gives well defined zones of inhibition. Freshly prepared plates should be used for antibiotic assays. This medium is widely employed as seed agar in the preparation of plates for microbiological agar diffusion assays.

Use: Recommended for microbiological assay of ß-lactam and other antibiotics as per the description of Indian Pharmacopoeia.

Ingredients:

Directions: Dissolve 30.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.