CAS Number
Formula
Weight
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Principle The media is composed according to the USP, recommended as antibiotic assay medium. It is composed of pancreatic digest of casein, papaic digest of soybean, sodium chloride, dibasic potassium phosphate, dextrose and agar. Pancreatic digest of casein and papaic digest of soybean provide nitrogen, carbon and other essential nutrients. Sodium chloride maintain osmatic equilibrium. Dibasic potassium phosphate resist change in pH. Dextrose serves as energy source. Agar is solidifying agent. The agar concentration is reduced for the proper diffusion of the antibiotic. The Polysorbate 80 enhances the penetration of antibiotic to the cytoplasmic membrane. Antibiotic Assay Medium No- 10 used as base agar for the antibiotic assay against the Pseudomonas aeruginosa and Bordetella bronchiseptia. |
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Use: Recommended for testing antibiotic potency against Pseudomonas aeruginosa for the antibiotics like Polymixin B, Carbenicillin, Colismethate, Colistin assay. |
Ingredients:
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Pancreatic Digest of Casein |
17,00 g/L |
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Papaic Digest of Soybean |
3,00 g/L |
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Sodium Chloride |
5,00 g/L |
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Dibasic Potassium Phosphate |
2,50 g/L |
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Dextrose |
2,00 g/L |
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Agar |
12,00 g/L |
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pH at 25°C |
7,2±0,2 |
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Formula adjusted for optimum performance and parameters |
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Directions: Dissolve 42.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely add 10 ml of Polysorbate 80. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.
Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.
Note: Sterilize media immediately after reconstitution.
Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.
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