DM50144 | Antibiotic Assay Medium No- 11

CAS Number

Formula

Weight

Principle:
 
The media is composed according to the USP, recommended as antibiotic assay medium. It is composed of
peptone, pancreatic digest of casein, yeast extract, meat extract, dextrose and agar. Peptone, pancreatic
digest of casein, yeast extract and meat extract provide nitrogen, carbon, vitamins essential nutrients.
Glucose serves as energy source. This medium is formulated in such a way to facilitate diffusion of the
antibiotics for antibiotic assay studies. Media is generally used of testing Neomycin, Erythromycin,
Kanamycin like antibiotics.
 

Ingredients:

Peptone

6,00 g/L

Panreatic Digest Casein

4,00 g/L

Yeast Extract

3,00 g/L

Meat Extract

1,50 g/L

Dextrose

1,00 g/L

Agar

15,00 g/L

pH at 25°C

8,30±0,2

Formula adjusted for optimum performance and parameters

Equivalent to beef extract

Directions:

Dissolve 30.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.

Storage:

Hygroscopic; Keep container tightly closed.

Store in cool (10- 30 °C) & Dry place.

For In Vitro Diagnostics.

For Laboratory, Institutional & Industrial use only.

Not for Human consumption.

Disposal:

To avoid the contamination or propagation of any hazardous microbes the used, unusable or

modified preparation of this product must be disposed after autoclaving after completion of task.

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