Products

DM50144 | Antibiotic Assay Medium No- 11

CAS Number

Formula

Weight

Principle:

The media is composed according to the USP, recommended as antibiotic assay medium. It is composed of

peptone, pancreatic digest of casein, yeast extract, meat extract, dextrose and agar. Peptone, pancreatic

digest of casein, yeast extract and meat extract provide nitrogen, carbon, vitamins essential nutrients.

Glucose serves as energy source. This medium is formulated in such a way to facilitate diffusion of the

antibiotics for antibiotic assay studies. Media is generally used of testing Neomycin, Erythromycin,

Kanamycin like antibiotics.

Ingredients:

Peptone	6,00 g/L
Panreatic Digest Casein	4,00 g/L
Yeast Extract	3,00 g/L
Meat Extract	1,50 g/L
Dextrose	1,00 g/L
Agar	15,00 g/L
pH at 25°C	8,30±0,2
Formula adjusted for optimum performance and parameters Equivalent to beef extract

Directions:

Dissolve 30.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.

Storage:

Hygroscopic; Keep container tightly closed.

Store in cool (10- 30 °C) & Dry place.

For In Vitro Diagnostics.

For Laboratory, Institutional & Industrial use only.

Not for Human consumption.

Disposal:

To avoid the contamination or propagation of any hazardous microbes the used, unusable or

modified preparation of this product must be disposed after autoclaving after completion of task.

Specifications

Dehydrated Powder
Description	A fine, free flowing, hygroscopic powder.
Color	Beige colored powder.
Solution
Solubility	Soluble in Distilled / de-ionized water at 50°C
Color	Light amber colored opalescent
pH	8,3 ±0,2 at 25°C0 30,50 g/ l)
Agar Plates
Solidification	40°C
Color	Light amber colored opalescent gel
Dehydrated Powder
Description	A fine, free flowing, hygroscopic powder.
Color	Beige colored powder.
Solution
Solubility	Soluble in Distilled / de-ionized water at 50°C
Color	Light amber colored opalescent
pH	8,3 ±0,2 at 25°C0 30,50 g/ l)
Agar Plates
Solidification	40°C
Color	Light amber colored opalescent gel
Organism	Recovery
Bacillus spizizenii @ATCC6633	≥70%
Dehydrated Powder
Description	A fine, free flowing, hygroscopic powder.
Color	Beige colored powder.
Solution
Solubility	Soluble in Distilled / de-ionized water at 50°C
Color	Light amber colored opalescent
pH	8,3 ±0,2 at 25°C0 30,50 g/ l)
Agar Plates
Solidification	40°C
Color	Light amber colored opalescent gel
Organism	Recovery/Antibiotic concentration (µg)/Zone of inhibition (mm)
Bacillus spizizenii @ATCC6633	≥70%/5/8

Products

DM50144 | Antibiotic Assay Medium No- 11

Specifications

Navigation