Products

DM50164 | Antibiotic Assay Medium No-19

CAS Number

Formula

Weight

Principle

The media is composed according to the USP, recommended as antibiotic assay medium. It is composed of

peptone, yeast extract, meat extract (equivalent to beef extract), sodium chloride, dextrose and agar.

Peptone, yeast extract and meat peptone provide nitrogen, carbon, long chain amino acids, vitamins and

essential nutrients for the growth of microorganisms. Sodium chloride maintains osmatic balance. Dextrose

serves as energy source. Agar is solidifying agent.

Use: Recommended for microbiological assay of antibiotics (Amphotericin B, Candicidin and Nystatin)

against variety of the microbes in accordance with United States Pharmacopoeia.

Ingredients:

Peptone	9,400
Yeast Extract	4,700
Meat Extract	2,400
Sodium Chloride	10,000
Dextrose	10,000
Agar	23,500
pH at 25°C	6,1 ±0,1
Formula adjusted for optimum performance and parameters

Directions: Dissolve 60.0 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C, and distribute aseptically in sterile petri plates. Ensure complete solidification and inoculate test organisms aseptically.

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

Specifications

Dehydrated Powder
Description	Light beige colored free flowing, homogeneous powder
Reaction of 6,0% solution	6,1 ±0,1 at 25℃
pH	5,90- 6,30
Gelling	Firm comparable with 2,35% agar gel
Color and clarity of ready medium	Light amber colored opalescent gel
Growth Promotion properties	Best at ≤ 100 CFU at 25-30⁰C for 24-72 h
Indicative properties	Optimum at ≤ 100 CFU at 25-30⁰C for 24-48 h
Negative control	Performed using sterile distilled water
Organism
Saccharomyces cerevisiae ATCC@9736	Inoculum/Growth/Recovery

Products

DM50164 | Antibiotic Assay Medium No-19

Specifications

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