CAS Number
Formula
Weight
|
Principle The media is composed according to the USP, recommended as antibiotic assay medium. It is composed of peptone, casein peptone, yeast extract, meat extract (equivalent to beef extract), dextrose, manganese sulphate and agar. Peptone, casein peptone, yeast extract and meat peptone provide nitrogen, carbon, long chain amino acids, vitamins and essential nutrients for the growth of microorganisms. Dextrose serves as energy source. Manganese sulphate in this medium facilitates the sporulation and growth of Bacillus subtilis. Agar is solidifying agent. |
|
Use: Recommended for microbiological assay of antibiotics (Dihydrostreptomycin and Vancomycin) against variety of the microbes especially Bacillus subtilis in accordance with United States Pharmacopoeia. |
Ingredients:
|
Peptone |
6,00 g/L |
|
Casein peptone |
4,00 g/L |
|
Yeast Extract |
3,00 g/L |
|
Meat Extract |
1,50 g/L |
|
Dextrose |
1,00 g/L |
|
Manganese Sulphate |
0,300 g/L |
|
Agar |
15,00 g/L |
|
pH at 25°C |
6,6±0,1 |
|
Formula adjusted for optimum performance and parameters |
|
|
Equivalent of Beef Extract |
|
Directions: Dissolve 30.80 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C, and distribute aseptically in sterile petri plates. Ensure complete solidification and inoculate test organisms aseptically.
Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.
Note: Sterilize media immediately after reconstitution.
Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.
Copyright © 2019 ICON CHEMICAL. All Rights Reserved.