CAS Number
Formula
Weight
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Principle |
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The media is composed according to the USP, recommended as antibiotic assay medium. It is composed of pancreatic digest of casein, papaic digest of soybean, sodium chloride, dibasic potassium phosphate, dextrose and agar. Peptone, pancreatic digest of casein and papaic digest of soybean provide nitrogen, carbon and essential nutrients. Sodium chloride maintains osmatic balance. Dibasic potassium phosphate acts as buffering agent. Dextrose serves as energy source. This medium is used as base layer for plate assay of antibiotics. Use: Recommended for microbiological assay of antibiotics (Carbenicillin, Colistimethate sodium, Colistin and Polymyxin B) against variety of the microbes in accordance with United States Pharmacopoeia. |
Ingredients:
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Pancreatic Digest of Casein |
17,00 g/L |
|
Papaic Digest of Soyabean |
3,00 g/L |
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Sodium Chloride |
5,00 g/L |
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Dibasic Potassium Phosphate |
2,50 g/L |
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Dextrose |
2,50 g/L |
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Agar |
20,00 g/L |
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pH at 25°C |
7,2±0,1 |
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Formula adjusted for optimum performance and parameters |
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Directions: Dissolve 50.0 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C, and distribute aseptically (21 ml) in petri plates to form a base layer. Ensure complete solidification and add 4 ml of seed layer of inoculum.
Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.
Note: Sterilize media immediately after reconstitution.
Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.
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