CAS Number
Formula
Weight
Usages:
For isolation and enumeration of coagulase-positive staphylococci in foodstuffs.
Principle:
Tryptone, beef extract powder and yeast extract powder provides carbon and nitrogen sources, vitamins and growth factors; sodium pyruvate and glycine to stimulate the growth of Staphylococcus aureus; lithium chloride and potassium tellurite inhibit the non-staphylococcal microorganisms; containing lecithinase staphylococcal colonies produce degradation yolk make transparent circle, while the role of the lipase produced an opaque precipitate ring; coagulase-positive staphylococci can restore potassium tellurite and produce black colonies; agar is medium coagulant.
Formulation(per liter):
Pancreatic Digest of Casein 10g
Beef Extract 5g
Yeast Extract 1g
Sodium Pyruvate 10g
Glycine 12g
Lithium Chloride 5g
Agar 20g
Final pH7.0 ± 0.2
How to use:
1.Suspend 63g in 950ML of distilled water , stirring heated to boiling ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
Quality control:
  
Item | The name and number of strain | Growth | Colony Color |
1 | Staphylococcus aureus ATCC6538 | Good | black |
2 | Staphylococcus epidermidis CMCC (B) 26069 | Good | Green-blue |
3 | Escherichia coli ATCC25922 | Not growth | -- |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
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