DM50015 | Baird-Parker Agar Base

CAS Number

Formula

Weight

Usages:
For isolation and enumeration of coagulase-positive staphylococci in foodstuffs.
 
Principle:
Tryptone, beef extract powder and yeast extract powder provides carbon and nitrogen sources, vitamins and growth factors; sodium pyruvate and glycine to stimulate the growth of Staphylococcus aureus; lithium chloride and potassium tellurite inhibit the non-staphylococcal microorganisms; containing lecithinase staphylococcal colonies produce degradation yolk make transparent circle, while the role of the lipase produced an opaque precipitate ring; coagulase-positive staphylococci can restore potassium tellurite and produce black colonies; agar is medium coagulant.
 
Formulation(per liter):
Pancreatic Digest of Casein 10g
Beef Extract 5g
Yeast Extract 1g
Sodium Pyruvate 10g
Glycine 12g
Lithium Chloride 5g
Agar 20g
Final pH7.0 ± 0.2
 
How to use:
1.Suspend 63g in 950ML of distilled water , stirring heated to boiling ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
 
Quality control:
            

Item The name and number of strain Growth Colony Color
1 Staphylococcus aureus ATCC6538 Good black
2 Staphylococcus epidermidis CMCC (B) 26069 Good Green-blue
3 Escherichia coli ATCC25922 Not growth --
 


Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
 

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