DM50015 | Baird-Parker Agar Base

CAS Number

Formula

Weight

Principle:

Baird Parker Agar was developed by Baird Parker (1962), composed of tryptone, meat extract (equivalent to beef extract), glycine, yeast extract, sodium pyruvate, lithium chloride and agar. Tryptone, meat extract and yeast extract provide nitrogen, carbon, sulphur and vitamins. Glycine and sodium pyruvate protect injured cells and helps to recovery them and stimulates the growth of Staphylococcus aureus. Lithium chloride and potassium tellurite acts as inhibitor agent for contaminating microflora. The tellurite additive is toxic to egg

yolk-clearing strains other than S. aureus and imparts a black color to the colonies.

Use: Recommended for the isolation, enrichment and enumeration of coagulase positive staphylococci from food and clinical samples.

 

Ingredients:

 

Tryptone

10,00 g/L

Meat Extract

5,00 g/L

Yeast Extract

1,00 g/L

Glycine

12,00 g/L

Sodium Pyruvate

10,00 g/L

Lithium Chloride

5,00 g/L

Agar

20,00 g/L

pH at 25°C

6,9±0,2

Formula adjusted for optimum performance and parameters

Equivalent to beef extract

 

Directions:

Dissolve 63.00 grams in 950 ml distilled water, boil to dissolve the medium completely and

sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C. After cooling to 45-50°C, add 50 mL of Egg Yolk Supplement and 3 ml sterile 3.5% Potassium Tellurite solution or 50 ml Egg Yolk Tellurite Emulsion. Mix thoroughly before dispensing.

 

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

 

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

 

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