Products

Principle

The medium contains protease peptone and infusions from calf brain and beef heart which serve as sources of carbon, nitrogen, essential growth factors, amino acids and vitamins. Dextrose is used as a source of energy. Disodium phosphate helps in maintaining the buffering action of the medium whereas sodium chloride maintains the osmotic equilibrium of the medium. Defibrinated sheep blood added to the basal medium provides essential growth factors for the more fastidious fungal organisms. It is a highly nutritious and can support luxuriant growth of wide variety of microorganisms. It can be further enriched by the addition of blood or rendered selective by adding different antibiotics. It is also used in the aminoglycoside and vancomycin screen test for resistant enterococci. BHA with 5-10% sheep blood and chloramphenicol

(16 μg/ml) and gentamicin (5 μg/ml) will inhibit the growth of bacteria while allowing growth of dimorphic fungi. This agar can be used as a primary plating medium. This medium used for primary isolation of aerobic bacteria from clinical specimens. BHI with 0.5% Polysorbate 80 can be used for detecting Mycobacterium avium-intracellular complex organisms and M. tuberculosis from blood cultures.

Use: Recommended for cultivation of fastidious pathogenic bacteria, yeasts and molds.

Beef Heart Infusion Form

250,00 g/L

Protease Peptone

10,00 g/L

Calf Brains Infusion From

200,00 g/L

Dextrose

2,00 g/L

Sodium Chloride

5,00 g/L

Disodium Phosphate

2.5,00 g/L

Agar

15,00 g/L

pH at 25°C

7,4±0,2

Formula adjusted for optimum performance and parameters