DM50023 | Brilliant Green Agar

CAS Number

Formula

Weight

Principle:

Brilliant green agar is composed of protease peptone, yeast extract, lactose, saccharose, sodium chloride, brilliant green, phenol red and agar. Protease peptone provides nitrogen and necessary growth elements. Yeast extract provide nitrogen, carbon, vitamins and other growth factors. Lactose and saccharose are source of carbohydrates. Sodium chloride maintains osmatic balance. Brilliant green inhibits both gram positive and negative bacteria except Salmonella species. Phenol red is pH indicator. Agar is solidifying agent.

 

Use: Recommended for isolating Salmonellae other than Salmonella typhimurium

 

Ingredients:

 

Proteose Peptone

10,00 g/L

Yeast Extract

3,00 g/L

Lactose

10,00 g/L

Saccharose

10,00 g/L

Sodium Chloride

5,00 g/L

Brilliant Green

0,012 g/L

Phenol Red

0,080 g/L

Agar

20,00 g/L

pH at 25°C

6,9±0,2

Formula adjusted for optimum performance and parameters

 

Directions:

Dissolve 58.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and

sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, (AVOID OVERHEATING) cool it to 42-45°C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.

 

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

 

Note: Sterilize media immediately after reconstitution.

 

Disposal:

To avoid the contamination or propagation of any hazardous microbes the used, unusable or

modified preparation of this product must be disposed after autoclaving after completion of task.

 

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