DM50026 | Bromcresol Purple Dextrose Broth

CAS Number

Formula

Weight

Usages:
For culturing bacteria and identification of bacterial fermentation of glucose and for  inspection of commercial sterilization of canned food.
 
Principle:
Peptone and beef extract powder provides carbon and nitrogen sources, vitamins and growth factors; fermentable sugars glucose; sodium chloride to maintain osmotic equilibrium; bromocresol purple as a pH indicator.
 
Formulation(per liter):
Peptone 10g
Beef extract powder 3g
Glucose 10g
Sodium chloride 5g
Bromocresol purple 0.04g
Final pH7.0 ± 0.2
        
 
How to use:
1.Suspend 28g in 1L of distilled water,stirring heated to boiling to completely dissolve ,autoclave at 100℃ for 30 minutes.
2.Diluted and treated samples.
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

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