DM50029 | Buffered Peptone Water (BPW)

CAS Number

Formula

Weight

Usages:
For pre-enrichment of Salmonella spp.
 
Principle:
Peptone provide carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride maintains osmotic equilibrium; potassium dihydrogen phosphate and disodium hydrogen phosphate as buffer.
 
Formulation(per liter):
Peptone: 10g
Sodium chloride:5g
Disodium hydrogen phosphate:3.5g
Potassium dihydrogen phosphate: 1.5g
Final pH7.2 ± 0.2
 
How to use:
1. Suspend 20g of product, adding 1L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 ℃ for 15min, set aside.
2.Diluted and treated samples.
 
Quality control:
            

Item The name and number of strain Growth Colony Color
1 Salmonella typhi CMCC (B) 50071 good Cloudy broth
2 Salmonella typhimurium CMCC (B) 50115 good Cloudy broth
3 Paratyphoid Salmonella CMCC (B) 50093 good Cloudy broth
 

 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

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