DM50029 | Buffered Peptone Water (BPW)

CAS Number

Formula

Weight

Principle:

Buffered peptone water is composed of peptone, sodium chloride, sodium phosphate dibasic and monobasic potassium phosphate. Peptone, serves the source of nitrogen, carbon and other growth factors. Dibasic sodium phosphate and monobasic potassium phosphate acts as buffering agents and sodium chloride maintains the osmotic balance. Buffered peptone water is designed to help recovery of the sub-healthy or damaged Salmonella from processed food samples before transferring to selective medium. Buffered peptone water has shown higher recovery of Salmonella than that of the Lactose broth.

Use: Recommended for enrichment of Salmonella from the pharmaceutical samples, water samples, food, dairy, animal feed and meat samples etc.

 

Ingredients:

 

Peptone

10,00 g/L

Sodium Chloride

5,00 g/L

Dibasic Sodium Phosphate

3,50 g/L

Monobasic Potassium Phosphate

1,50 g/L

pH at 25°C

7,2±0,2

Formula adjusted for optimum performance and parameters

 

Directions:

Dissolve 20.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely.

Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, and inoculate test sample aseptically.

 

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

 

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