Products

Principle:

Cetrimide agar is described by King et.al (1954) and is prepared in accordance with the harmonized principles of USP/EP/IP. Recommended as selective medium for the isolation of Pseudomonas aeruginosa in pharmaceutical testing and microbial limit testing of pharmaceutical products and raw material used in pharmaceutical industries. Media consists of pancreatic digest of gelatin, magnesium chloride, dipotassium sulfate, cetrimide and agar. The pancreatic digest of gelatin and peptone provides essential nutrients, vitamins and nitrogenous factors and growth factors required for growth of microorganisms. The magnesium chloride and potassium sulphate stimulate pyocyanin and fluorescein production. Cetrimide is the selective agent and inhibits most bacteria by acting as a detergent. The cetrimide came in contact with bacteria, causes the release of nitrogen and phosphorous from the bacterial cell other than Pseudomonas aeruginosa. Glycerol (not provided) is supplemented as a source of carbon. Agar is the solidifying agent.

Use: Recommended for the isolation of Pseudomonas aeruginosa from pharmaceutical products in accordance with the microbial limit testing by harmonized principles of USP/EP/IP.

Ingredients:

Directions:

Dissolve 45.50 grams in 1000 ml distilled water containing 1% glycerol, boil to dissolve the

medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45°C and distribute aseptically in sterile petri plates and allow to solidify. Ensure complete solidification and inoculate test sample aseptically.

Storage and Shelf Life:

The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when

stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.