Products

Principle

L-Cystine lactose electrolyte deficient medium composed of peptone, tryptone, meat extract, lactose, L-Cystine and bromothymol blue. Literature suggests that the Proteus species can be controlled by restricting the electrolytes and by replacing the mannitol by lactose and sucrose with L-Cystine and bromothymol blue.  Peptone, meat extract and tryptone serve as the source of all essential nutrients such as amino acids, vitamins, other trace factors. L-Cystine is added as a growth supplement for cystine-dependent coliforms. Lactose is included as a carbon source and plays a crucial role for selection of lactose fermenting microbes. Brom Thymol Blue is used as a pH indicator. Organisms capable of fermenting lactose will lower the pH of medium, result in change the color of the medium from green to yellow. Agar is used as a solidifying agent.

Use: Recommended for cultivating, differentiating and enumerating lactose fermenting bacteria in urine.

Meat Extract

3,00 g/L

Peptone

4,00 g/L

Tryptone

4,00 g/L

Lactose

10,00 g/L

L-Cystine

0,128 g/L

Bromothymol Blue

0,020 g/L

Agar

15,00 g/L

pH at 25°C

7,3±0,2

Formula adjusted for optimum performance and parameters

Equivalent of Beef extract