DM50048 | Eosin Methylene Blue Agar (EMB Agar)

CAS Number

Formula

Weight

Usages:
For separating Gram-negative enterobacteria especially coliform and fecal coliform.
 
Principle:
Peptone provide carbon and nitrogen; lactose is coliform fermentable sugars; dipotassium phosphate as a buffer; agar as culture medium coagulant; eosin beauty blue is bacteriostatic agents and pH indicator, inhibits leather Gram-positive bacteria.
 
Formulation(per liter):
Peptone 10g
Lactose 10g
Dipotassium hydrogen phosphate 2g
Agar 15g
Eosin Y 0.4g
Methylene 0.065g
Final pH 7.1 ± 0.2
 
How to use:
1.Suspend 37.5g in 1L of distilled water. Heat with frequent agitation and boil to completely dissolve. Distribute into flasks. Autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Navigation

Copyright © 2019 ICON CHEMICAL. All Rights Reserved.