Products

Principle:

Eosin methylene blue agar composed of peptone, lactose, sucrose, dipotassium phosphate, Eosin Y, methylene blue and agar. Peptone as a source of carbon, nitrogen, vitamins and minerals. Lactose and sucrose are fermentable carbohydrates. Dipotassium phosphate act as buffer. Eosin and methylene blue are dyes provide selectivity to media and act as pH indicator and inhibitors. The media also differentiate between the lactose fermenters and non-lactose fermenters. Some gram-negative bacteria can not able to metabolize lactose or metabolize lactose very slowly. Hence, the sucrose is used as secondary carbohydrate

source. While the lactose non-fermenters such as Salmonella sp. raise the pH of surrounding by deamination of protein thus resulting in change of pH, solubilize the Eosin Methyl red complex and grow as colorless colonies. While the coliforms or lactose fermenters produce acid and decrease pH abound the colonies, result in precipitation of both the dyes at acidic ph. Produce black purple colonies, such as Escherichia coli forms characteristic green colored metallic sheen. Gram-positive bacteria are partially inhibited on the medium. Agar is a solidifying agent.

Use: Recommended for the isolation, enumeration and differentiation of members of Enterobacteriaceae from clinical and non-clinical samples.

Ingredients:

Directions:

Dissolve 36.00 grams in 1000 ml distilled water check. Boil to dissolve the medium completely

and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute aseptically. Ensure complete solidification and inoculate test sample aseptically.

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal:

To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.