DM50050 | Fluid Tetrationate Broth (TTB)

CAS Number

Formula

Weight

Usages:
For salmonella selective enrichment and cultivation.
 
Principle:
Peptone provide carbon, nitrogen and vitamins to meet the needs of bacterial growth; calcium carbonate can neutralize bacterial acid production and absorption of toxic metabolites; bovine bile salts inhibit E. coli and other Gram-positive bacilli.
 
Formulation(per liter):
Peptone 5g
Ox bile salt 1g
Calcium carbonate 10g
Sodium thiosulfate 30g
Final pH 8.4 ± 0.2
 
How to use:
1.Suspend 46g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min.
2.Diluted and treated samples.
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

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