CAS Number
Formula
Weight
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Principle Lauryl sulphate broth is composed of tryptose, lactose, potassium phosphate dibasic and monobasic, sodium chloride and sodium lauryl sulfate. Tryptose provides nitrogen and amino acids. Lactose is fermentable carbohydrate. Potassium phosphates are buffering agents. Sodium chloride maintain osmatic balance. Sodium lauryl sulfate inhibits non-coliforms. The selectivity to the medium is provide by lactose and sodium lauryl sulfate. |
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Use: Recommended for detecting coliform organisms in water and wastewater. |
Ingredients:
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Tryptose |
20,00 g/L |
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Lactose |
5,00 g/L |
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Potassium Phosphate Dibasic |
2,750 g/L |
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Potassium Phosphate Monobasic |
2,750 g/L |
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Sodium Chloride |
5,00 g/L |
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Sodium Lauryl Sulfate |
0,100 g/L |
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pH at 25°C |
6,8±0,2 |
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Formula adjusted for optimum performance and parameters |
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Directions: Dissolve 35.60 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute in test tube containing inverted Durham’s tube. Sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
Note: for 1.0 or less than 1.0 ml of water sample single strength broth is used and for more than 1.0 ml double strength broth is used.
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Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label. Note: Sterilize media immediately after reconstitution. |
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Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task. |
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