Products

Principle:

Luria Bertani agar is modification of LB broth, Miller, composed of tryptone, yeast extract, sodium chloride and agar. The Tryptone serves the source of nitrogen and amino acids, yeast extract provides nitrogen and vitamins and other growth factors. Sodium chloride maintains the osmotic equilibrium. Agar is a solidifying agent. The Luria Bertani (Miller) is used for the cultivation of Escherichia coli.

Use: Recommended for cultivation of recombinant strains of Escherichia coli.

Ingredients:

Directions:

Dissolve 40.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and

sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.

Storage and Shelf Life:

The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when

stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal:

To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.