Products

Principle:

Luria Bertani broth, Miller, composed of tryptone, yeast extract and sodium chloride. The Tryptone serves the source of nitrogen and amino acids, yeast extract provides nitrogen and vitamins and other growth factors. Sodium chloride maintains the osmotic equilibrium. The Luria Bertani (Miller) is used for the cultivation of Escherichia coli.

Use: Recommended for cultivation of recombinant strains of Escherichia coli.

Ingredients:

Directions: Dissolve 25.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.