Products

Principle:

MacConkey broth is composed of peptone, proteose peptone, lactose, bile salt, sodium chloride and neutral red. Peptone and proteose peptone provide nitrogen and other nutrients necessary for the growth of microorganism. Lactose is a carbon source and plays a important role for selection of lactose fermenting microbes. Bile salt is selective agent, inhibit growth of gram-positive organisms. Neutral red is pH indicator dye. The medium turns pink in case of lactose fermenters and yellow in case of non-lactose fermenters.

Use: Recommended for enrichment and enumeration of lactose fermenting coliforms from pharmaceutical, clinical and non-clinical samples, dairy, food and water samples.

Ingredients:

Directions:

Dissolve 41.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute in test tubes containing inverted Durham's tube. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.    

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.