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Principle

MacConkey agar is composed of peptone, proteose peptone, lactose, bile salt, sodium chloride, neutral red, crystal violet and agar. Peptone and proteose peptone provide nitrogen and other nutrients necessary for the growth of microorganism. Lactose is a carbon source and plays a important role for selection of lactose fermenting microbes. Bile salt and crystal violet are selective agents, inhibit growth of gram-positive organisms. Neutral red is pH indicator dye. Agar is solidifying agent. When lactose is fermented, acid is produced and result in bile salt precipitation.  Since the lactose fermenting colonies of coliform bacteria are pink in color and may be surrounded by a zone of bile precipitation. While the bacteria that do not ferment lactose remain colorless.

Use: Recommended by Ph. Eur., ISO-21567, for isolation and differentiation of lactose fermenting and lactose non-fermenting gram-negative enteric bacilli from pharmaceutical, clinical and non-clinical samples, dairy, food and water samples.

Peptone

17,00 g/L

Proteose Peptone

3,00 g/L

Lactose

10,00 g/L

Bile Salt

1,50 g/L

Sodium Chloride

5,00 g/L

Neutral Red

0,030 g/L

Crystal Violet

0,001 g/L

Agar

13,50 g/L

pH at 25°C

7,1±0,2

Formula adjusted for optimum performance and parameters