Products

Principle

Mannitol salt agar is a medium used for the detection and enumeration of Staphylococci. It was described by Chapman and prepared in accordance with the harmonized principles of USP/IP/JP as medium for microbial limit testing of pharmaceutical products and raw material used in pharmaceutical industries. Mannitol salt agar contains pancreatic digest of casein, peptic digest of animal tissue and meat extract (equivalent to beef extract) as sources of carbon, nitrogen, vitamins and minerals. Sodium chloride, in high concentration (7.5%), act as selective agent, allow only to grow the Staphylococci and halophilic Enterobacteria and inhibit other bacteria. Phenol red is the pH indicator. D-Mannitol is the carbohydrate source. Mannitol fermentation with an accumulation of acid products, detected by the phenol red indicator which turns yellow and produces a yellow halo surrounding the presumptive pathogen colonies. Characteristic non-pathogenic Staphylococci do not ferment mannitol and form red colonies. Since, this medium exploits the correlation between the pathogenic and fermentative capacity of mannitol of staphylococci, for a presumptive diagnosis.

Use: Recommended for selective isolation and detection of Staphylococci according USP/IP/JP as medium for microbial limit testing of pharmaceutical products.

Pancreatic Digest of Casein

5,00 g/L

Peptic Digest of Animal Tissue

5,00 g/L

Meat Extract

1,00 g/L

D-Mannitol

10,00 g/L

Sodium Chloride

75,00 g/L

Phenol Red

0,025 g/L

Agar

15,00 g/L

pH at 25°C

7,4±0,2

Formula adjusted for optimum performance and parameters

Equivalent to beef extract

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.