Products

Principle:

MR VP broth contains buffered peptone as a carbon and nitrogen source for general growth requirements.

Dextrose is a fermentable carbohydrate. Dipotassium phosphate is buffering agent. Some Enterobacteriaceae

convert glucose to pyruvate by the Embden-Meyerhof pathway. While other bacteria metabolize pyruvate by

the mixed acid pathway and produce acidic end products, such as lactic, acetic and formic acids. The acid so

produced decreases the pH to 4.5 or below, which is indicated by a change in the color of methyl red from

yellow to red. The glucose metabolizing and stabile acid producing bacteria are detected by the methyl red

test (MR test), If the bacteria have the ability to utilize glucose with production of a stable acid, the color of

the methyl red changes from yellow to red. While the other bacteria metabolize pyruvate by the butylene

glycol pathway and produce neutral end products), one of which is acetoin (acetylmethylcarbinol). If acetyl

methyl carbinol is produced, react ∝- naphthol, strong alkali (40% KOH), and atmospheric oxygen,

converted to diacetyl. The diacetyl and quinidine containing compounds found in the peptones of the

medium condense to form a pinkish red polymer.

Use: Recommended for the Methyl Red and Voges-Proskauer tests in differentiation of the coli-aerogenes

group.

Ingredients: