Products

Principle

Mueller Hinton agar with 2% glucose and methylene blue is the modification of formula recommended by Mueller and Hinton (1941) for the primary isolation of Neisseria species. Commonly it is used for performing antibiotic susceptibility tests using a disc diffusion assay and due to its susceptibility for microorganism and high reproducibility; WHO Committee recommended it for standardization of susceptibility testing. Media is simple and composed of meat infusion form (equivalent of beef infusion form), Casamino acids, starch, glucose, methylene blue and agar. Meat Infusion from and casamino acids provides nitrogen vitamins, amino acids, minerals, carbon and other nutrients to support the growth of microorganisms. While starch absorb any toxic metabolites produced during microbial growth and while autoclaving undergoes hydrolysis and liberate small amount of dextrose, which act as source of energy. The additional glucose serves as an energy source for fungal cultures while Methylene blue enhances zone edge definition. Agar is the solidifying agent.

Use: Recommended for testing performing antifungal disk diffusion susceptibility of yeasts.

Meat Infusion Form

300,00 g/L

Casamino Acids

17,50 g/L

Starch

1,50 g/L

Glucose

20,00 g/L

Methylene blue

0,0005 g/L

Agar

17,00 g/L

pH at 25°C

7,3±0,2

Formula adjusted for optimum performance and parameters

Equivalent to beef extract

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.