DM50084 | Mueller Hinton Broth (MH Broth)

CAS Number

Formula

Weight

Principle

Mueller Hinton Medium duplicates the formula recommended by Mueller and Hinton (1941) for the primary isolation of Neisseria species. Currently its widely used for performing antibiotic susceptibility tests using the tube dilution method. Media is simple and composed of meat infusion form (equivalent of beef infusion form), Casamino acids and starch. Infusion from Meat and Casamino acids provides nitrogen vitamins, amino acids, minerals, carbon and other nutrients to support the growth of microorganisms. While starch absorb any toxic metabolites produced during microbial growth and while autoclaving undergoes hydrolysis and liberate small amount of dextrose, which act as source of energy.

Use: Recommended for antimicrobial susceptibility testing of rapidly growing aerobic and facultative anaerobic microbes by the tube dilution method.

Ingredients:

 

Meat Infusion Form

300,00 g/L

Casamino Acids

17,50 g/L

Starch

1,50 g/L

pH at 25°C

7,3±0,2

Formula adjusted for optimum performance and parameters

Equivalent to beef extract

Directions: Dissolve 21.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically. 

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

 

 

 

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