CAS Number
Formula
Weight
Principle:
Nutrient agar is a basic medium and commonly used for cultivation, enrichment, enumeration, prolonged maintenance and storage of wide variety of microbes. The nutrient agar is widely used as non-selective microbial medium for routine microbiology analysis. Nutrient agar is prominently used for cultivation of the non-fastidious organisms. Nutrient agar is ideal microbial media for the teaching, demonstration and routine analysis. Nutrient Agar is a simple medium composed of the peptone, meat extract (equivalent to beef extract), yeast extract which provide ample amount of the carbon and nitrogen essential and nonessential amino acids, along with the vitamins and trace minerals. Sodium chloride maintains the osmotic equilibrium of the medium. Agar is solidifying agent.
Use: Recommended for the refinement of non-fastidious microorganisms from clinical and non-clinical samples. It is generally used for examination of water and dairy products.
Ingredients:
|
Peptone |
10,00 g/L |
|
Meat Extract |
5,00 g/L |
|
Yeast Extract |
1,00 g/L |
|
Sodium Chloride |
12,00 g/L |
|
Agar |
20,00 g/L |
|
pH at 25°C |
6,9±0,2 |
|
Formula adjusted for optimum performance and parameters |
|
|
Equivalent to beef extract |
|
Directions: Dissolve 28.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in desired. Ensure complete solidification and inoculate test sample aseptically.
Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.
Note: Sterilize media immediately after reconstitution.
Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.
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