DM50086 | Nutrient Broth(NB)

CAS Number

Formula

Weight

Principle

Nutrient broth is a basic media and commonly used for cultivation, enrichment, enumeration, prolonged maintenance and storage of wide variety of microbes. The nutrient broth is widely used as non-selective microbial media for routine microbiology analysis and cultivation of non-fastidious organisms. Nutrient broth is ideal microbial media for teaching, demonstration and routine analysis. Nutrient Broth (NB) is a simple medium composed of the peptone, meat extract (Equivalent to beef extract), yeast extract and sodium chloride. Peptone, meat extract and yeast extract provide ample amount of the carbon and nitrogen essential and non-essential amino acids, along with the vitamins and trace minerals. Sodium chloride maintains the osmotic equilibrium of the medium.

Use: Recommended for the refinement of non-fastidious microorganisms from clinical and non-clinical samples. Nutrient broth is ideal microbial media for teaching, demonstration and routine analysis.

Ingredients:

Peptone

5,00 g/L

Meat Extract

1,50 g/L

Yeast Extract

1,50 g/L

Sodium Chloride

5,00 g/L

pH at 25°C

7,4±0,2

Formula adjusted for optimum performance and parameters

Equivalent to beef extract

Directions: Dissolve 13.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute aseptically. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.   

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

 

 

 

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