DM50086 | Nutrient Broth(NB)

CAS Number

Formula

Weight

Usages:
For cultivating and enriching nofastidious bacteria ,and for evaluating the efficacy of disinfection.
 
Principle:
Peptone and beef extract powder provide nitrogen, vitamins, amino acids and carbon; sodium chloride to maintain osmotic equilibrium.
 
Formulation(per liter):
Peptone 10.0g
Beef Extract 3.0g
Sodium Chloride 5.0g
Final pH 7.4 ± 0.2
 
How to use:
1.Suspend 18g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
 
Quality control:
            

Item The name and number of strain Growth Colony Color
1 Escherichia coli ATCC25922 Good Turbid broth,slight precipitate
2 Staphylococcus aureus ATCC25922 Good Turbid broth,slight precipitate
 

 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

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