Products

Principle:

Potato dextrose agar is composed of potato infusion, dextrose and agar. Potato infusion, serve as the source of all essential nutrients such as amino acids, vitamins, other trace elements, required for the growth of yeast and molds. Dextrose supply carbon and energy source. The potato infusion is also known to encourages the sporulation and pigment production in some fungal species. The acidic pH makes the medium partially

selective for the growth of fungi and inhibit the growth of bacteria.

Use: Recommended for culturing yeasts and molds from food and dairy products.

Ingredients:

Directions:

Dissolve 39.00 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs. Pressure (121 °C) for 15 min, cool it to 42-45 °C. Distribute aseptically in desired and allow to solidify. Ensure complete solidification and inoculate test sample aseptically.

For more selectivity to fungi, reduce the pH of medium to 3.6 to inhibit bacterial growth. Dissolve 39.00 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 45-50 °C. Add 10% sterile tartaric acid (approximately 1ml) till pH reaches to 3.6, mix well and distribute in desired (avoid reheating the medium). Ensure complete solidification and inoculate test sample.

Storage and Shelf Life:

The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal:

To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.