Products

Principle

The Rappaport Vassiliadis Salmonella Enrichment broth is the modification of the R10 medium (Rappaport et.al. 1956) or RV broth (Vassiliadis et.al. 1978), designed by Van Schothorst et al. (1987). Recommended by USP and JP for microbial limit testing medium for pharmaceutical products especially for Salmonella species. The media composed of soya peptone, magnesium chloride, sodium chloride, dipotassium phosphate, potassium dihydrogen phosphate, and malachite green. Soya peptone serves carbon and nitrogen sources for growth requirements. Magnesium chloride raises the osmotic pressure in the medium. The malachite green and magnesium chloride together act as selective agent and inhibit the growth of other organisms than Salmonellae. The low pH of the medium (5.2±0.2 at 25°C), combined with the presence of malachite green and magnesium chloride, provides selectivity to highly resistant Salmonella spp. The phosphate buffers the medium.

Use: Recommended for selective enrichment of Salmonella species from pharmaceutical products in accordance with the microbial limit testing by harmonized principles of USP/JP.

Soya Peptone

4,50 g/L

Magnesium Chloride

29,00 g/L

Sodium Chloride

8,00 g/L

Dipotassium phosphate

0,400 g/L

Potassium Dihydrogen Phosphate

0,600 g/L

Malachite Green

0,036 g/L

pH at 25°C

5,2±0,2

Formula adjusted for optimum performance and parameters

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.