Products

Principle:

Reinforced medium for clostridia is used for enumerating and cultivating Clostridia. It is also recommended by USP, IP and EP for microbial limit testing of pharmaceutical products and raw material used in pharmaceutical industries. Media is composed of meat extract ( equivalent to beef extract), peptone, yeast extract, soluble starch, glucose monohydrate, cysteine hydrochloride, sodium chloride, sodium acetate and small quantity of agar. Meat extract, peptone, yeast extract, starch, and cysteine hydrochloride provide nutrients and co-factors required for growth of Clostridia. In addition to that cysteine hydrochloride acts as reducing agent and soluble starch absorb toxic metabolites from the medium. Glucose monohydrate is included in the medium as an energy source. Sodium chloride maintains osmotic equilibrium. Sodium acetate acts as buffering agent. Less quantity of agar makes the medium semi solid and helps in maintaining anaerobic conditions.

Ingredients:

Directions:

Dissolve 38.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute aseptically in test tubes. Allow to cool and inoculate test sample aseptically.

Storage:

Hygroscopic; Keep container tightly closed. Store in cool (10- 30 ?) & Dry place (<40%). For Laboratory, Institutional & Industrial use only. Not for Human consumption.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving or incineration after completion of task.

Expected Performance when stored at optimum conditions and within the expiry date.

No liability accepted for accidents in handling or use.