Products

Principle

Rose Bengal agar base is composed of soya peptone, dextrose, monopotassium phosphate, magnesium sulfate, rose bengal and agar. Soya peptone provides nitrogen and amino acids and other trace nutrients required for the growth of microorganisms. Dextrose is an energy source. Monopotassium phosphate acts as buffering agent. Magnesium sulfate provides ions for metabolic reactions. Rose Bengal is a selective agent, inhibits bacterial growth and prevent over growth of fastidious fungi and aids in isolation of slow growing fungi. The rose Bengal is absorbs by the yeast and molds and help in identification and enumeration. Agar is solidifying agent. The media can be fortified with chloramphenicol, which inhibits bacteria.

Use: Recommended for isolation and enumeration of yeast and molds.

Soya Peptone

5,00 g/L

Dextrose

10,00 g/L

Monopotassium Phosphate

1,00 g/L

Magnesium Sulfate

0,50 g/L

Rose Bengal

0,05 g/L

Agar

15,00 g/L

pH at 25°C

7,2±0,2

Formula adjusted for optimum performance and parameters

Directions: Dissolve 32.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.  

Note: For more selectivity add 2 ml of chloramphenicol solution (0.1 g in 2ml of ethanol) in cooled agar base mix thoroughly and dispense in sterile petri plates.

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.