DM50153 | Salmonella Shigella Agar

CAS Number

Formula

Weight

Principle:

Salmonella Shigella agar composed of proteose peptone, meat extract, lactose, bile salts, sodium citrate, sodium thio-sulphate, ferric citrate, neutral red, brilliant green and agar. Proteose peptone and meat extract provide essential growth nutrients. Lactose is the fermentable carbohydrate. Brilliant green, bile salts and thiosulphate provide selectively and inhibit gram-positive and coliform organisms. Certain organisms reduce sodium thiosulphate to sulphite and H2S gas. Sodium thiosulfate and ferric citrate allow the detection of hydrogen sulfide by the production of colonies with black centers. Neutral red and brilliant green are present as pH indicators.

Use: Recommended for the selective isolation and determination of Salmonella and some Shigella species from clinical and non-clinical samples.

Ingredients:

 

Proteose Peptone

5,00 g/L

Meat Extract

5,00 g/L

Lactose

10,00 g/L

Bile Salt Mixture

8,50 g/L

Sodium Citrate

8,50 g/L

Sodium thio-Sulphate

8,50 g/L

Ferric Citrate

1,00 g/L

Neutral Red

0,025 g/L

Brilliant Green

0,033 g/L

Agar

13,50 g/L

pH at 25°C

5,1±0,2

Formula adjusted for optimum performance and parameters

Equivalent to beef extract

 

Directions: Dissolve 60.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely for sterilization. DO NOT AUTOCLAVE, cool it to 42-45 °C and distribute aseptically in to the plates. Ensure complete solidification and inoculate aseptically.

 

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

 

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