CAS Number
Formula
Weight
Principle:
SIM Medium used for differentiation of enteric bacteria on basis of three different characteristics, H2S Production, motility and Indole production. The media is composed of special peptone, peptone, peptonized iron, sodium thiosulphate and agar. Peptone and special peptone provide nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other essential nutrients. Special peptone contains tryptophan, which is degraded by specific bacteria to produce Indole. The Indole is detected by the addition of chemical reagents following the incubation period. Peptonized iron and sodium thiosulphate are the indicators of H2S production. This H2S reacts with peptonized iron to form black precipitate of ferrous sulphide. Motile organisms exaggerate the H2S reaction. Motile organisms grow away from line of inoculation showing diffused growth while non-motile organisms grow along the stab line. Motility detection is possible due to the semisolid nature of the medium. Growth radiating out from the central stab line indicates that the test organism is motile.
Use: Recommended for determination of hydrogen sulphide production, indole formation and motility of enteric bacilli from clinical and non-clinical samples.
Ingredients:
|
Special Peptone |
3,00 g/L |
|
Peptone |
30,00 g/L |
|
Peptonized Iron |
0,20 g/L |
|
Sodium thiosulphate |
0,025 g/L |
|
Agar |
3,00 g/L |
|
pH at 25°C |
7,3±0,2 |
|
Formula adjusted for optimum performance and parameters |
|
Directions: Dissolve 36.225 grams in 1000 ml purified / distilled water. Heat to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Distribute aseptically in test tubes and allow to cool to 40°C in an upright position.
Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.
Note: Sterilize media immediately after reconstitution.
Disposal: To avoid the contamination or propagation of any hazardous microbes used, unusable or modified preparation of this product must be disposed after autoclaving or incineration after completion of task.
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