Products

Principle

Simmons Citrate Agar is a modification of Koser’s medium (1923). Simmons citrate agar is composed of magnesium sulphate, ammonium dihydrogen phosphate, dipotassium phosphate, sodium citrate, sodium chloride, bromothymol blue and agar. Magnesium sulfate is a cofactor for variety of metabolic reactions for growth and reproduction of microorganisms. Ammonium dihydrogen phosphate is the source of nitrogen. Dipotassium Phosphate acts as a buffer. Sodium Citrate is source of carbon. Sodium Chloride maintains the osmotic balance. Bromothymol blue act as pH indicator. Agar is solidifying agent. The microorganisms have ability to utilize citrate as a source of energy and capable of utilizing ammonium dihydrogen phosphate will grow on this medium. The citrate metabolizing microbes accumulate alkaline by products by synthesizing citrate permease, which convert citrate to pyruvate. The synthesized pyruvate is used by the organisms in metabolic cycle for energy production. The citrate metabolizing bacteria, break down the ammonium slats into the ammonia, result rise in alkalinity of the medium. The shift in pH turns the bromothymol blue indicator in the medium from green to blue above pH 7.6. Simmons citrate agar is recommended by APHA and BIS for the differentiation between Enterobacteriaceae.

Use: Recommended for differentiation the members of Enterobacteriaceae on the basis of citrate utilization.

Magnesium Sulphate

0,20 g/L

Ammonium Dihydrogen Phosphate

1,00 g/L

Dipotassium Phosphate

1,00 g/L

Sodium Citrate

2,00 g/L

Sodium Chloride

5,00 g/L

Bromothymol Blue

0,080 g/L

Agar

15,00 g/L

pH at 25°C

6,8±0,1

Formula adjusted for optimum performance and parameters

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.