DM50125 | Tryptic Soy Agar(TSA)

CAS Number

Formula

Weight

Principle:

Tryptone soya agar is used for cultivation of a wide variety of organisms. The medium is a highly nutritious composed of tryptone, soya peptone, sodium chloride and agar. Tryptone, papain digest of soy peptone provides ample amount of the carbon and nitrogen source and trace. Sodium chloride maintains the osmotic balance and agar is used as solidifying agent.

 

Use: Recommended for the isolation and cultivation of fastidious microorganisms from clinical and nonclinical samples.

 

Ingredients:

 

Tryptone

15,00 g/L

Soya Peptone

5,00 g/L

Sodium Chloride

5,00 g/L

Agar

15,00 g/L

pH at 25°C

7,3±0,2

Formula adjusted for optimum performance and parameters

 

Directions: Dissolve 40.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates and allow to solidify. Ensure complete solidification and inoculate test sample aseptically.

 

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

 

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

 

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