DM50127 | Tryptic Soy Broth (TSB)

CAS Number

Formula

Weight

Principle:

Tryptic Soy Broth is composed of tryptone, soy peptone, dextrose, sodium chloride and dipotassium phosphate. Tryptone and soy peptone provide nitrogen and other essential nutrients. Dextrose is a carbon source, serves as energy source for optimum growth. Sodium chloride maintains osmotic balance, while dipotassium phosphate is a buffering agent. It is general purpose medium used for cultivation of fastidious and non-fastidious organisms.

 

Use: Recommended for cultivation of wide variety of microorganism.

 

Ingredients:

 

Tryptone

17,00 g/L

Soy Peptone

3,00 g/L

Dextrose

2,50 g/L

Sodium Chloride

5,00 g/L

Dipotassium Phosphate

2,50 g/L

pH at 25°C

7,3±0,2

Formula adjusted for optimum performance and parameters

 

Directions:

Dissolve 30.00 grams in 1000 ml distilled water; boil to dissolve the medium completely and

distribute aseptically. Sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.

 

Storage and Shelf Life:

The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

 

Disposal:

To avoid the contamination or propagation of any hazardous microbes the used, unusable or

modified preparation of this product must be disposed after autoclaving after completion of task.

 

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