DM50133 | Urea Agar Base

CAS Number

Formula

Weight

Principle:
Urea agar base is composed of peptone, dextrose, sodium chloride, monobasic potassium phosphate, phenol red and agar. Peptone provides carbon and nitrogen required for the growth of organisms. Dextrose is the energy source. Sodium chloride maintains the osmotic equilibrium and phosphates act as buffering agent. Many urinary tract infections causing organisms, produce urease enzyme, which catalyzes the splitting of urea in the presence of water to release two molecules of ammonia and carbon dioxide. The ammonia combines with the carbon dioxide and water to form ammonium carbonate, which turns the medium alkaline, and change the color of medium from light orange at pH 6.8 to magenta pink at pH 8.1 due to presence of phenol red. Rapid urease-positive organisms turn the entire medium pink within 24 hours.

Use: Recommended for detection of the urease positive microbes from clinical and environmental samples.

Ingredients:

 

Peptone

1,00/L

Dextrose

1,00g/L

Sodium Chloride

5,00g/L

Disodium hydrogen phosphate

1,20g/L

Potassium dihydrogen phosphate

0,800g/L

Phenol Red

0.012 g/L

Agar

15.00 g/L

pH at 25?C

6,8  ±0,2

 

Directions: Dissolve 24.01 grams in 950 ml distilled water, boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C and add 50 ml of sterile 40% Urea solution mix well and distribute in sterile test tubes. Slant the tubes to have a small butt and slant, allow to solidify. Ensure complete solidification and inoculate test sample aseptically.

 

Specimens types analyzed

Pharmaceutical samples, clinical and non-clinical samples etc.

 

Precautions to be taken

All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

 

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