Products

Principle

Violet red bile lactose agar is recommended by APHA (1978) and ISO (1991). Violet red bile lactose agar contains yeast extract, pancreatic digest of gelatin, bile salts, sodium chloride, lactose, neutral red, crystal violet and agar. The pancreatic digest of gelatin serves as a source of carbon, nitrogen, vitamins and minerals. Yeast Extract supplies additional nitrogen, carbon and vitamins which stimulate bacterial growth. Lactose is a source of carbohydrate. Bile salts and crystal violet perform inhibitory action and inhibit gram positive bacteria. The fermentation of lactose to acid is indicated by the pH indicator neutral red, which changes its color to red, and by precipitation of bile acids. Organisms that rapidly attack lactose produce red purple colonies surrounded by purple haloes. While the non-fermenters or late lactose-fermenters produce pale colonies with greenish zones. Agar is a solidifying agent.

Use: Recommended for selective isolation, detection and enumeration of coli-aerogenes bacteria in food and water, milk other dairy food products and clinical samples.

Yeast Extract

3,00 g/L

Pancreatic Digest of Gelatin

7,00 g/L

Bile Salts

1,50 g/L

Sodium Chloride

5,00 g/L

Lactose

10,00 g/L

Neutral Red

0,030 g/L

Crystal Violet

0,002 g/L

Agar

15,00 g/L

pH at 25°C

7,4±0,2

Formula adjusted for optimum performance and parameters

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.