Products

Principle

Vogel-Johnson agar medium (VJ medium) is composed of tryptone, yeast extract, mannitol, dipotassium phosphate, lithium chloride, glycine, phenol red and agar. Tryptone and yeast extract provide nitrogenous and carbonaceous compounds, long chain amino acids, vitamin B complex and other growth nutrients. Mannitol is source of carbon. Dipotassium phosphate acts as buffering agent. Lithium chloride and glycine inhibits most of microorganisms except staphylococci. Phenol red is pH indicator. Agar is solidifying agent.

The addition of potassium tellurite 1% provides more selectivity to the medium for isolation of staphylococci. After 24 hours of incubation, most of contaminating organisms are almost inhibited by tellurite, lithium chloride and high glycine content. Staphylococcus aureus also be inhibited by these inhibitors but get compensated by mannitol and glycine. VJ Agar selects and differentiates the coagulase positive staphylococci which ferment mannitol and reduce potassium tellurite.

Use: Recommended for selective isolation of mannitol fermenting Staphylococcus aureus from heavily contaminated food and clinical specimens (after addition of potassium tellurite).

Tryptone

10,00 g/L

Yeast Extract

5,00 g/L

Mannitol

10,00 g/L

Dipotassium Phosphate

5,00 g/L

Lithium Chloride

5,00 g/L

Glycine

10,00 g/L

Phenol Red

0,025 g/L

Agar

15,00 g/L

pH at 25°C

7,2±0,2

Formula adjusted for optimum performance and parameters

Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label.

Note: Sterilize media immediately after reconstitution.

Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.