DM50138 | W.L. Nutrient Agar

CAS Number

Formula

Weight

Usages:
For cultivation and examination of microorganisms.
 
Principle:
Yeast extract ,tryptone provide carbon and nitrogen sources, vitamins and growth factors.
 
Formulation(per liter):
Yeast Extract 4g
Tryptone   5g
Glucose   50g
Agar   20g
Potassium Dehydrogen Phosphate  0.55g
Calcium Chloride 0.425g
Magnesium Sulphate 2.5g
Ferric Chloride   2.5g
Bromocresol Green  0.022g
 
Final pH 5.5 ± 0.2
  
 
How to use:
1.Suspend 80g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min.
2.Diluted and treated samples.
 
Quality control:
     

Item The name and number of strain Growth Colony Color
1 Escherichia coli ATCC25922 Good White
2 Staphylococcus aureus ATCC6538 Good Yellow
 

      
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

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