M200097 | IconCut® EcoRV

CAS Number

Formula

Weight

IconCut® EcoRV is expressed and purified from E.coli that carries the recombinant EcoRV gene. The molecular weight is 29.5 kDa, with the recognition site at GAT^ATC. The reaction is conducted for 5-15 minutes at 37?, and heat-inactivated at 65? for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
 

Recognition Site
5’...GAT↓ATC...3’
3’...CTA↑TAG...5’
 

Applications
Genomic DNA, plasmid DNA, PCR product
 

Storage
at -20°C  for two years
 

Shipping
Dry ice (-70 ?)

 

 

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