CAS Number
Formula
Weight
IconCut® PstI is expressed and purified from E.coli that carries the recombinant PstI gene. The molecular weight is 38.3 kDa, with the recognition site at CTGCA^G. The reaction is conducted for 5-15 minutes at 37?, and heat-inactivated at 80? for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...CTGCA↑G...3’
3’...G↑ACGTC...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ?)
Copyright © 2019 ICON CHEMICAL. All Rights Reserved.