CAS Number
Formula
Weight
IconCut® SphI is expressed and purified from E.coli that carries the recombinant SphI gene. The molecular weight is 26.8 kDa, with the recognition site at GCATG^C. The reaction is conducted for 5-15 minutes at 37?, and heat-inactivated at 65? for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...GCATG↓C...3’
3’...C↑GTACG...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ?)
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