Products

Unique genomic DNA remover is combined with IconScript® II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis at 42-55°C. After cDNA synthesis, gDNA remover and IconScript® II RT/RI Enzyme Mix are inactivated by heating at 85°C for 5 seconds.
 

• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.

• After reaction, heat inactivation for RT/RI and gDNA remover simultaneously can avoid damage to RNA caused by heat inactivating DNase I used for pretreatment in traditional protocol.
• Simple and fast procedure.

• cDNA up to 15 kb.
 

Applications
• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
 

Storage
at -20 ? for two years
 

Shipping
Dry ice (-70 ?)