Products

IconStart®  FastPfu Fly DNA Polymerase is a hot start, ultra high-fidelity DNA Polymerase used for fast PCR. Compared with IconStart®  FastPfu DNA Polymerase, IconStart®  FastPfu Fly DNA Polymerase has higher extension rate (≤ 5 kb fragments can achieve 12 kb/min extreme amplification, > 5 kb fragments can achieve 6 kb/min high-speed amplification), featuring higher amplification efficiency, higher yield, higher fidelity and higher specificity. 2×IconStart®  FastPfu Fly Reaction Mix already contains dNTPs. When DNA is amplified, just add template, primer, water and IconStart®  FastPfu Fly DNA Polymerase. The Reaction can be carried out with the concentration of Reaction Mix being 1×. PCR products are blunt end and can be cloned directly into pIcon®-Blunt series of vectors.

• Offers 108-fold fidelity as compared to IconTaq® DNA Polymerase.

• PCR products can be directly cloned into pIcon ^®-Blunt vectors.

• Amplification of genomic DNA fragment up to 15 kb.

• Amplification of plasmid DNA fragment up to 20 kb.

Applications

• Complex templates, GC/AT-rich templates.

• Ultra high-fidelity and fast PCR, blunt end cloning, site-directed mutagenesis.

• Long fragment amplification.

Storage
at -20 ? for two years
 

Shipping
Dry ice (-70 ?)