Products

IconTaq® DNA Polymerase for PAGE is purified from E. Coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. IconTaq® DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
 

• Extension rate is about 1-2 kb/min.
• Unique buffer system compatible with PAGE.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pIcon®-Tvectors.
• Amplification of genomic DNA fragment up to 3 kb.
 

Applications
Short fragment PCR
 

Storage
at -20 ? for two years
 

Shipping
Dry ice (-70 ?)