Products

IconTaq® DNA Polymerase High Fidelity (IconTaq®HiFi DNA Polymerase) contains IconTaq®-T DNA Polymerase and a proofreading 3’-5’ exonuclease.IconTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than IconTaq®-T DNA Polymerase. Two different buffers are provided in the kit.IconTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and IconTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
 

• IconTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared to IconTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pIcon®-Tvectors.
• Amplification of genomic DNA fragment up to 15 kb.
 

Applications
• Complex templates
• GC/AT rich templates
• Long PCR
• High yield PCR
 

Storage
at -20 ? for two years
 

Shipping
Dry ice (-70 ?)