CAS Number
Formula
Weight
IconTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. IconTaq® DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. IconTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pIcon®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.
Applications
• Routine PCR
• High throughput PCR
• Colony PCR
Storage
at -20 ? for two years
Shipping
Dry ice (-70 ?)
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